Optimized Workflow for Multiplexed Phosphorylation Analysis of TMT-Labeled Peptides Using High-Field Asymmetric Waveform Ion Mobility Spectrometry
نویسندگان
چکیده
منابع مشابه
Miniaturized ultra high field asymmetric waveform ion mobility spectrometry combined with mass spectrometry for peptide analysis.
Miniaturized ultra high field asymmetric waveform ion mobility spectrometry (ultra-FAIMS) combined with mass spectrometry (MS) has been applied to the analysis of standard and tryptic peptides, derived from α-1-acid glycoprotein, using electrospray and nanoelectrospray ion sources. Singly and multiply charged peptide ions were separated in the gas phase using ultra-FAIMS and detected by ion tra...
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High-field asymmetric waveform ion mobility spectrometry (FAIMS) is shown to be capable of resolving isomeric and isobaric glycosaminoglycan negative ions and to have great utility for the analysis of this class of molecules when combined with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) and tandem mass spectrometry. Electron detachment dissociation (EDD) and other ion...
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The analysis of intact glycopeptides by mass spectrometry is challenging due to the numerous possibilities for isomerization, both within the attached glycan and the location of the modification on the peptide backbone. Here, we demonstrate that high field asymmetric wave ion mobility spectrometry (FAIMS), also known as differential ion mobility, is able to separate isomeric O-linked glycopepti...
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Liquid extraction surface analysis (LESA) is a surface sampling technique that allows electrospray mass spectrometry analysis of a wide range of analytes directly from biological substrates. Here, we present LESA mass spectrometry coupled with high field asymmetric waveform ion mobility spectrometry (FAIMS) for the analysis of dried blood spots on filter paper. Incorporation of FAIMS in the wor...
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a) Department of Chemistry and Biochemistry ([email protected]), New Mexico State University, Las Cruces, NM 88003 USDA-ARS-SW Cotton Ginning Research Lab ([email protected]), 300 E. College Drive, PO Box 578, Mesilla Park, NM 88047 c) SIONEX Corporation ([email protected]), 300 Second Ave., Waltham, MA 02451 Discrimination of combustion fuel sources using gas chromatography-planar field asymmetr...
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ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2019
ISSN: 1535-3893,1535-3907
DOI: 10.1021/acs.jproteome.9b00759